Hatching of Echinostoma trivolvis miracidia in response to snail host and non-host chemical cues

Abstract

Environmental cues are used by many organisms to time life history transitions and can be important for trematode host location. However, while much is understood about how larval trematodes locate hosts, much less is known about the potential role of host cues in the timing of trematode egg development and hatching. We addressed the potential role of host chemical cues in mediating hatching of Echinostoma trivolvis miracidia by comparing hatching in response to cues from the first intermediate host (the snail Planorbella trivolvis), a non-host snail (the snail Goniobasis proxima), and a non-host invertebrate (earthworm, Lumbricus terrestris). We hypothesized that in the presence of cues from their first intermediate host, E. trivolvis would hatch sooner and would be more synchronized than when host cues were absent. However, we found that hatching was unaffected by our cue treatments. In all treatments, hatching uniformly began at 13 days and was nearly evenly spread over the next 3 weeks.