Purification of herpesvirus nucleocapsids by fluorocarbon extraction
- PMID: 195183
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Purification of herpesvirus nucleocapsids by fluorocarbon extraction
Microbios.
1976.
Abstract
Purified nucleocapsids were prepared from herpes simplex virus type-1 and herpes simplex virus type-2 infected cells, using a fluorocarbon extraction technique with Freon 113. Fifty to 85% of the total nucleocapsid population remained 'nucleated' or full in terms of the nucleic acid core making them useful as a source of DNA. CsCl banded DNA represented 30--40% of the theoretical yield of the total nucleocapsid population.
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