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. 2009 Jul;6(7):503-6.
doi: 10.1038/nmeth.1338. Epub 2009 Jun 14.

Quantitative analysis of gene expression in a single cell by qPCR

Kiyomi Taniguchi  1 , Tomoharu Kajiyama, Hideki Kambara
Affiliations

Quantitative analysis of gene expression in a single cell by qPCR

Kiyomi Taniguchi et al. Nat Methods. 2009 Jul.

Abstract

We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or less. This method is sufficiently accurate to investigate the heterogeneity of single cells.

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