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Review
. 2010 Jan;46(1):101-12.
doi: 10.1007/s10858-009-9331-1. Epub 2009 Jun 16.

Paramagnetic labelling of proteins and oligonucleotides for NMR

Xun-Cheng Su  1 , Gottfried Otting
Affiliations
Review

Paramagnetic labelling of proteins and oligonucleotides for NMR

Xun-Cheng Su et al. J Biomol NMR. 2010 Jan.

Erratum in

  • J Biomol NMR. 2011 May;50(1):99-100

Abstract

Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.

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References

    1. Biochemistry. 2003 Jul 8;42(26):8011-21 - PubMed
    1. J Am Chem Soc. 2006 Mar 22;128(11):3696-702 - PubMed
    1. FEBS Lett. 1999 Nov 5;460(3):519-26 - PubMed
    1. Protein Sci. 2008 Jul;17(7):1220-31 - PubMed
    1. Ann N Y Acad Sci. 1973 Dec 31;222:163-74 - PubMed

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