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. 2009 Jul;38(6):807-12.
doi: 10.1007/s00249-009-0501-6. Epub 2009 Jun 18.

Structured illumination microscopy of a living cell

Liisa M Hirvonen  1 , Kai Wicker, Ondrej Mandula, Rainer Heintzmann
Affiliations

Structured illumination microscopy of a living cell

Liisa M Hirvonen et al. Eur Biophys J. 2009 Jul.

Abstract

Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.

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