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. 2009 Sep;119(5):783-93.
doi: 10.1007/s00122-009-1088-0. Epub 2009 Jun 21.

Integrated genetic map and genetic analysis of a region associated with root traits on the short arm of rye chromosome 1 in bread wheat

Sundrish Sharma  1 , Prasanna R Bhat, Bahman Ehdaie, Timothy J Close, Adam J Lukaszewski, J Giles Waines
Affiliations

Integrated genetic map and genetic analysis of a region associated with root traits on the short arm of rye chromosome 1 in bread wheat

Sundrish Sharma et al. Theor Appl Genet. 2009 Sep.

Abstract

A rye-wheat centric chromosome translocation 1RS.1BL has been widely used in wheat breeding programs around the world. Increased yield of translocation lines was probably a consequence of increased root biomass. In an effort to map loci-controlling root characteristics, homoeologous recombinants of 1RS with 1BS were used to generate a consensus genetic map comprised of 20 phenotypic and molecular markers, with an average spacing of 2.5 cM. Physically, all recombination events were located in the distal 40% of the arms. A total of 68 recombinants was used and recombination breakpoints were aligned and ordered over map intervals with all the markers, integrated together in a genetic map. This approach enabled dissection of genetic components of quantitative traits, such as root traits, present on 1S. To validate our hypothesis, phenotyping of 45-day-old wheat roots was performed in five lines including three recombinants representative of the entire short arm along with bread wheat parents 'Pavon 76' and Pavon 1RS.1BL. Individual root characteristics were ranked and the genotypic rank sums were subjected to Quade analysis to compare the overall rooting ability of the genotypes. It appears that the terminal 15% of the rye 1RS arm carries gene(s) for greater rooting ability in wheat.

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Figures

Fig. 1
Fig. 1
Polymerase chain reaction amplicon from 1BS-specific dominant marker Xucr_4 (575 bp) among different wheat lines on 1.2% agarose gel elctrophoresis. The numbers at the top of panels correspond to lines carrying different lengths of 1RS chromatin in wheat backgrounds. 1B + lines are 1BS arm with distal 1RS chromatin and T-lines are 1RS arm with distal wheat chromatin
Fig. 2
Fig. 2
Diagrammatic representation of genetic positions of recombinant breakpoints of 1RS-1BS in Pavon 76 bread wheat. White bars are representing wheat 1BS and black bars represent rye 1RS. On the left side of the figure is the list of markers present starting from the centromere at the bottom (represented by circle; white = wheat and black = rye centromeres) to the top toward telomeres. Each black and white bar represents the 1RS-1BS arm showing the position of the markers with respect to recombinant breakpoints and these bars represent only the 40% distal end of the chromosome
Fig. 3
Fig. 3
Physical distribution of recombinant breakpoints on the short arm of chromosome 1R-1B along the centromere (0), telomere (t) axis
Fig. 4
Fig. 4
Integrated genetic map of the rye–wheat 1RS-1BS arm showing the genetic distances (in cM) between markers. Genetic map represents 40% of the distal end of 1RS and 1BS chromosomes
See this image and copyright information in PMC

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