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. 2011:2011:474026.
doi: 10.1093/ecam/nep039. Epub 2011 Jun 16.

Evaluation of the Wound Healing Potential of Achillea biebersteinii Afan. (Asteraceae) by In Vivo Excision and Incision Models

Esra Küpeli Akkol  1 , Ufuk Koca, Ipek Pesin, Demet Yilmazer
Affiliations

Evaluation of the Wound Healing Potential of Achillea biebersteinii Afan. (Asteraceae) by In Vivo Excision and Incision Models

Esra Küpeli Akkol et al. Evid Based Complement Alternat Med. 2011.

Abstract

Achillea species are widely used for diarrhea, abdominal pain, stomachache and healing of wounds in folk medicine. To evaluate the wound healing activity of the plant, extracts were prepared with different solvents; hexane, chloroform, ethyl acetate and methanol, respectively from the roots of Achillea biebersteinii. Linear incision by using tensiometer and circular excision wound models were employed on mice and rats. The wound healing effect was comparatively evaluated with the standard skin ointment Madecassol. The n-hexane extract treated groups of animals showed 84.2% contraction, which was close to contraction value of the reference drug Madecassol (100%). On the other hand the same extract on incision wound model demonstrated a significant increase (40.1%) in wound tensile strength as compared to other groups. The results of histoptological examination supported the outcome of linear incision and circular excision wound models as well. The experimental data demonstrated that A. biebersteinii displayed remarkable wound healing activity.

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Figures

Figure 1
Figure 1
Effects of the extracts from A. biebersteinii on circular excision wound model.
Figure 2
Figure 2
Effects of the extracts from A. biebersteinii on linear incision wound model.
Figure 3
Figure 3
Microscopic view of the section of negative control group (untreated) 10 days old wound tissue. (a) area of ulceration; (b) mixed type inflammatory cells; (c) edema and (d) congested vessel.
Figure 4
Figure 4
Microscopic view of the section of 10 days old wound tissue treated with n-hexane extract. (a) hair follicle; (b) fibroblast; (c) collagen fibres and (d) blood vessel.
Figure 5
Figure 5
Microscopic view of the section of 10 days old wound tissue treated with Reference material Madecassol® (a) intact epidermis; (b) keratinization; (c) collagen fibers and (d) hair follicle.
Figure 6
Figure 6
The hypothetical diagram demonstrating the possible effect of the extract of A. biebersteinii in wound healing activity.
See this image and copyright information in PMC

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