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Comparative Study
. 2009 Aug;47(8):2662-6.
doi: 10.1128/JCM.02415-08. Epub 2009 Jun 24.

Use of a new single multiplex PCR-based assay for direct simultaneous characterization of six Neisseria meningitidis serogroups

Christophe Fraisier  1 , Richard Stor, Bernard Tenebray, Yannick Sanson, Pierre Nicolas
Affiliations
Comparative Study

Use of a new single multiplex PCR-based assay for direct simultaneous characterization of six Neisseria meningitidis serogroups

Christophe Fraisier et al. J Clin Microbiol. 2009 Aug.

Abstract

We developed a new Neisseria meningitidis multiplex PCR to determine six serogroups, including X-specific primers, and to allow direct W135/Y discrimination. This assay offers a simple and low-cost method for serogrouping N. meningitidis from cerebrospinal fluid that could be useful in Africa.

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Figures

FIG. 1.
FIG. 1.
Multiplex PCR amplification of the siaD/synB and siaD/synE (serogroups B and C), synG and synF (serogroups W135 and Y), orf-2 (serogroup A), and ctrA (serogroup X) genes from samples belonging to serogroup A (lanes 1 to 8), B (lanes 9 to 17), C (lanes 18 to 21) (A) and X (lanes 22 to 31) and W135 (lanes 32 to 43) and Y (lanes 44 to 50) (B). DNA from other species, Haemophilus influenzae (lanes 51, 52, 54, 55, 57) and Streptococcus pneumoniae (lanes 53, 56, 58, 59, and 60), were used as negative controls. PCR products were separated on a 4% agarose gel. Molecular sizes are indicated.
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