Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2009 Jun 25;113(26):6503-5.
doi: 10.1182/blood-2009-04-214320.

Functional neutrophils from human ES cells

Colin L Sweeney  1 , Harry L Malech
Affiliations
Comment

Functional neutrophils from human ES cells

Colin L Sweeney et al. Blood. .

Abstract

In this issue of Blood, Yokoyama and colleagues demonstrate in vitro differentiation of hESCs into mature neutrophils with functional capabilities (chemotaxis, phagocytosis, microbicidal oxidase activity, and bacterial killing) approaching or equal to that of normal peripheral blood neutrophils.

PubMed Disclaimer

Conflict of interest statement

Conflict-of-interest disclosure: The authors declare no competing financial interests. ■

Figures

None
Treatment of genetic neutrophil disorders using patient-derived induced pluripotent stem cells (iPSCs) capable of producing functional neutrophils. Mature cells (fibroblasts or other cell types) could be obtained from the patient and reprogrammed into iPSCs using defined reprogramming factors. Following gene correction or repair, iPSCs could be expanded and differentiated in vitro using the Yokoyama et al or another similar protocol, modified to provide hematopoietic stem cells that are capable of producing functional neutrophils after autologous transplantation. Professional illustration by Marie Dauenheimer.
See this image and copyright information in PMC

Comment on

References

    1. Yokoyama Y, Suzuki T, Sakata-Yanagimoto M, et al. Derivation of functional mature neutrophils from human embryonic stem cells. Blood. 2009;113:6584–6592. - PubMed
    1. Saeki K, Saeki K, Nakahara M, et al. A feeder-free and efficient production of functional neutrophils from human embryonic stem cells. Stem Cells. 2009;27:59–67. - PubMed
    1. Wang L, Menendez P, Shojaei F, et al. Generation of hematopoietic repopulating cells from human embryonic stem cells independent of ectopic HOXB4 expression. J Exp Med. 2005;201:1603–1614. - PMC - PubMed
    1. Tian X, Woll PS, Morris JK, et al. Hematopoietic engraftment of human embryonic stem cell-derived cells is regulated by recipient innate immunity. Stem Cells. 2006;24:1370–1380. - PubMed
    1. Ledran MH, Krassowska A, Armstrong L, et al. Efficient hematopoietic differentiation of human embryonic stem cells on stromal cells derived from hematopoietic niches. Cell Stem Cell. 2008;3:85–98. - PubMed