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. 2009 Oct 30;27(46):6420-3.
doi: 10.1016/j.vaccine.2009.06.023. Epub 2009 Jun 24.

Production of yellow fever virus in microcarrier-based Vero cell cultures

Marta Cristina O Souza  1 , Marcos S Freire, Erica A Schulze, Luciane P Gaspar, Leda R Castilho
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Free article

Production of yellow fever virus in microcarrier-based Vero cell cultures

Marta Cristina O Souza et al. Vaccine. .
Free article

Abstract

In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor to evaluate the use of a commercial serum-free medium (VP-SFM) and to investigate the effects of multiplicity of infection (MOI) and time of infection (TOI) on virus production. Virus titers as high as 8.4 x 10(8)pfu/mL were obtained upon infection with MOI of 0.02 and TOI of 3 days, using the serum-free medium in the sparged bioreactor.

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