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. 2008 Jan;1(1):2-9.
doi: 10.4103/0974-1208.38973.

Screening of 'Y' chromosome microdeletions in Iranian infertile males

Ali Mohammad Malekasgar  1 , Hayat Mombaini
Affiliations

Screening of 'Y' chromosome microdeletions in Iranian infertile males

Ali Mohammad Malekasgar et al. J Hum Reprod Sci. 2008 Jan.

Abstract

Background: It has been hypothesized that microdeletions of Yq may account for a significant proportion of men with infertility. Three nonoverlapping regions, referred to as "azoospermia factors" (AZFa, b, c from proximal to distal Yq) have been defined as spermatogenesis loci and deletions in these regions have been shown to be pathogenically involved in male infertility associated with azoospermia or severe oligospermia.

Aims: Evaluation the frequency of Y chromosome microdeletions in Iranian population.

Materials and methods: Fifty infertile men were selected. Semen analysis was done and on the basis of the mean sperm count, all patients were categorized into azoospermia and oligozoospermia, groups. Blood samples were obtained for DNA extraction and chromosomal analysis. Genomic DNA was extracted from blood lymphocytes and amplified by sequence tagged sites-polymerase chain reaction (STS-PCR) method to determine the presence of microdeletions in AZF locus. A total of 34 STS primers including two controls were selected to identify microdeletions of Y chromosome on each subject.

Results and conclusion: 26/50 cases (52%) showed deletion of at least one of the STS Marker. Totally 41 microdeletions was observed. A total of 17 cases (34%) had deletion in one STS. Four oligospermia cases (8%) had deletion in 2 STS site. Three azoospermia cases (6%) had again deletion in 2 STS site, but in different STSs. One case had three deletions in three STS site and finally one individual had seven deletions in AZF locus. The overall frequency of Y chromosome microdeletions observed in the present study was found to be 26/50 (52%). Comparison of our data with the result of other investigators world wide shows that the incidence of Yq microdeletions in Iranian population is much higher than international frequency. Our data agree with other studies regarding microdeletions of AZFc, but for microdeletions of AZFa (14.6%) our results is much higher and differ significantly with many studies.

Keywords: Azoospermia; Y-chromosome microdeletions; infertility; oligospermia.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Y chromosome
Figure 2
Figure 2
Electroforesis results of PCR product amplified by sY182 primer in some patients. Patient's number is indicated at top. Arrows represent the deleted subjects. MC, male control; FC, female control
Figure 3
Figure 3
Electroforesis results of multiplex PCR product amplified by sY283 primer in patient 34 with SRY control. Patients’ numbers are indicated at top. Arrows represent the deleted subjects. MC, male control; FC, female control
Figure 4
Figure 4
Electroforesis results of multiplex PCR product amplified by sY182 primer in patients’ numbers 2, 5, 9, 12, 22, and 49. Patients’ numbers are indicated at top. Arrows represent the deleted subjects. MC, male control; FC, female control
See this image and copyright information in PMC

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