Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 May-Jun;14(3):030501.
doi: 10.1117/1.3127202.

Imaging of glioma tumor with endogenous fluorescence tomography

Dax S Kepshire, Summer L Gibbs-Strauss, Julia A O'Hara, Michael Hutchins, Niculae Mincu, Frederic Leblond, Mario Khayat, Hamid Dehghani, Subhadra Srinivasan, Brian W Pogue

Imaging of glioma tumor with endogenous fluorescence tomography

Dax S Kepshire et al. J Biomed Opt. 2009 May-Jun.

Erratum in

  • J Biomed Opt. 2009 May-Jun;14(3):039802. Gibbs-Strauss, Summer L [corrected to Gibbs-Struass, Summer L]

Abstract

Tomographic imaging of a glioma tumor with endogenous fluorescence is demonstrated using a noncontact single-photon counting fan-beam acquisition system interfaced with microCT imaging. The fluorescence from protoporphyrin IX (PpIX) was found to be detectable, and allowed imaging of the tumor from within the cranium, even though the tumor presence was not visible in the microCT image. The combination of single-photon counting detection and normalized fluorescence to transmission detection at each channel allowed robust imaging of the signal. This demonstrated use of endogenous fluorescence stimulation from aminolevulinic acid (ALA) and provides the first in vivo demonstration of deep tissue tomographic imaging with protoporphyrin IX.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The fluorescence tomography system photograph is shown in (a), with the main components labeled. A tomography dataset as measured through a tissue phantom, and (b) the reconstructed image data is overlayed on this, and in (c) the recovered image from the phantom is shown.
Figure 2
Figure 2
The reconstructed quantum yield multiplied by the fluorophore absorption coefficient is shown within the region of the phantom, for a range of increasing PpIX concentrations. The linearity of the recovery provides a reliable way to calibrate the concentration from within tomographic images.
Figure 3
Figure 3
Image of the rodent glioma tumor, as viewed by contrast magnetic resonance imaging (a), and the fluorescence tomography image of PpIX in (b), and the overlay of this image onto the MicroCT image of the cranium slice (c).
See this image and copyright information in PMC

References

    1. Ntziachristos V, Tung CH, Bremer C, Weissleder R. Fluorescence molecular tomography resolves protease activity in vivo. Nature Medicine. 2002;8(7):757–60. - PubMed
    1. Choi HK, Yessayan D, Choi HJ, Schellenberger E, Bogdanov A, Josephson L, Weissleder R, Ntziachristos V. Quantitative analysis of chemotherapeutic effects in tumors using in vivo staining and correlative histology. Cellular Oncology. 2005;27(3):183–90. - PMC - PubMed
    1. Ntziachristos V, Schellenberger EA, Ripoll J, Yessayan D, Graves E, Bogdanov A, Jr., Josephson L, Weissleder R. Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V-Cy5.5 conjugate. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(33):12294–9. - PMC - PubMed
    1. Ntziachristos V. Fluorescence molecular imaging. Annual Review of Biomedical Engineering. 2006;8:1–33. - PubMed
    1. Deliolanis N, Lasser T, Hyde D, Soubret A, Ripoll J, Ntziachristos V. Free-space fluorescence molecular tomography utilizing 360 degrees geometry projections. Optics Letters. 2007;32(4):382–4. - PubMed

Publication types