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. 2009 Jul 2;5(1):87-96.
doi: 10.1016/j.stem.2009.05.025.

Induction of pluripotency in adult unipotent germline stem cells

Kinarm Ko  1 , Natalia Tapia, Guangming Wu, Jeong Beom Kim, Marcos J Araúzo Bravo, Philipp Sasse, Tamara Glaser, David Ruau, Dong Wook Han, Boris Greber, Kirsten Hausdörfer, Vittorio Sebastiano, Martin Stehling, Bernd K Fleischmann, Oliver Brüstle, Martin Zenke, Hans R Schöler
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Free article

Induction of pluripotency in adult unipotent germline stem cells

Kinarm Ko et al. Cell Stem Cell. .
Free article

Abstract

Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germline-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses, gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.

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