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Clinical Trial
. 2010 Sep;94(4):1314-1319.
doi: 10.1016/j.fertnstert.2009.05.019. Epub 2009 Jul 8.

Replete vitamin D stores predict reproductive success following in vitro fertilization

Sebiha Ozkan  1 , Sangita Jindal  2 , Keri Greenseid  2 , Jun Shu  3 , Gohar Zeitlian  2 , Cheryl Hickmon  2 , Lubna Pal  4
Affiliations
Clinical Trial

Replete vitamin D stores predict reproductive success following in vitro fertilization

Sebiha Ozkan et al. Fertil Steril. 2010 Sep.

Abstract

Objective: To determine whether 25OH-D levels in the follicular fluid (FF) of infertile women undergoing IVF demonstrate a relationship with IVF cycle parameters and outcome, hypothesizing that levels of 25OH-D in body fluids are reflective of vitamin repletion status.

Design: Prospective cohort study.

Setting: Academic tertiary care center.

Patient(s): Eighty-four infertile women undergoing IVF.

Intervention(s): Follicular fluid from follicles>or=14 mm; serum (n = 10) and FF levels of 25OH-D.

Main outcome measure(s): Clinical pregnancy (CP), defined as evidence of intrauterine gestation sac on ultrasound, following IVF; IVF cycle parameters.

Result(s): Serum and FF levels of 25OH-D were highly correlated (r=0.94). In a predominantly Caucasian population (66%), significantly lower FF 25OH-D levels were noted in Black versus non-Black patients. Significant inverse correlations were seen between FF 25OH-D levels and body mass index (r=-0.25). Significantly higher CP and implantation rates were observed across tertiles of FF25OH-D; patients achieving CP following IVF (n=26) exhibited significantly higher FF levels of 25OH-D. Multivariable logistic regression analysis confirmed FF 25OH-D levels as an independent predictor to success of an IVF cycle; adjusting for age, body mass index, ethnicity, and number of embryos transferred, each ng/mL increase in FF 25OH-D increased the likelihood for achieving CP by 6%.

Conclusion(s): Our findings that women with higher vitamin D level in their serum and FF are significantly more likely to achieve CP following IVF-embryo transfer are novel. A potential for benefit of vitamin D supplementation on treatment success in infertile patients undergoing IVF is suggested and merits further investigation.

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Figures

Figure 1
Figure 1
Follicular fluid 25-OH Vitamin D reliably reflects serum levels
Figure 2
Figure 2
Population prevalence (%) of Vitamin D status
Figure 3
Figure 3
Significantly higher follicular fluid 25OH Vitamin D levels are noted in IVF cycles achieving clinical pregnancy
Figure 4
Figure 4
Increasing implantation and clinical pregnancy rates are observed across FF 25OHD tertiles.
See this image and copyright information in PMC

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