doi: 10.1016/0014-4827(91)90430-3.
Establishment of a germ-line competent C57BL/6 embryonic stem cell line
Affiliations
- PMID: 1959560
- DOI: 10.1016/0014-4827(91)90430-3
Item in Clipboard
Establishment of a germ-line competent C57BL/6 embryonic stem cell line
Exp Cell Res.
1991 Dec.
Abstract
Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of the male ES cell lines tested (BL/6-III) was shown to be karyotypically stable and germ-line competent when introduced into BALB/c host blastocysts. These results demonstrate that ES cell lines from inbred mouse strains other than 129/Sv may be used as vectors to introduce selected mutations into the germ-line of mice.
Similar articles
-
Strain difference in establishment of mouse embryonic stem (ES) cell lines.Int J Dev Biol. 1994 Jun;38(2):385-90. Int J Dev Biol. 1994. PMID: 7981049
-
Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media.Genesis. 2004 Jun;39(2):100-4. doi: 10.1002/gene.20031. Genesis. 2004. PMID: 15170695
-
The origin and efficient derivation of embryonic stem cells in the mouse.Proc Natl Acad Sci U S A. 1997 May 27;94(11):5709-12. doi: 10.1073/pnas.94.11.5709. Proc Natl Acad Sci U S A. 1997. PMID: 9159137 Free PMC article.
-
New approach for the establishment of mouse early embryonic stem cells and induction of their differentiation.Hum Cell. 2001 Dec;14(4):283-91. Hum Cell. 2001. PMID: 11925930 Review.
-
Embryonic germ cell lines and their derivation from mouse primordial germ cells.Ciba Found Symp. 1994;182:157-68; discussion 168-78. doi: 10.1002/9780470514573.ch9. Ciba Found Symp. 1994. PMID: 7835148 Review.
Cited by
-
Mouse genetics in the 21st century: using gene targeting to create a cornucopia of mouse mutants possessing precise genetic modifications.Cytotechnology. 1993;11(2):79-99. doi: 10.1007/BF00748997. Cytotechnology. 1993. PMID: 7763692 Review.
-
Gene targeting in mice: a review.Methods Mol Biol. 2013;1064:315-36. doi: 10.1007/978-1-62703-601-6_23. Methods Mol Biol. 2013. PMID: 23996268 Free PMC article. Review.
-
Embryonic stem cells and iPS cells: sources and characteristics.Vet Clin North Am Equine Pract. 2011 Aug;27(2):233-42. doi: 10.1016/j.cveq.2011.04.003. Epub 2011 Jun 16. Vet Clin North Am Equine Pract. 2011. PMID: 21872756 Free PMC article.
-
Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background.World J Stem Cells. 2009 Dec 31;1(1):22-9. doi: 10.4252/wjsc.v1.i1.22. World J Stem Cells. 2009. PMID: 21607104 Free PMC article.
-
Permissive role of thrombopoietin and granulocyte colony-stimulating factor receptors in hematopoietic cell fate decisions in vivo.Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):698-702. doi: 10.1073/pnas.96.2.698. Proc Natl Acad Sci U S A. 1999. PMID: 9892696 Free PMC article.
MeSH terms
LinkOut - more resources
Other Literature Sources
Medical
Molecular Biology Databases
Research Materials