doi: 10.1083/jcb.74.2.358.
Binding of viral glycoprotein mRNA to endoplasmic reticulum membranes is disrupted by puromycin
- PMID: 195964
- PMCID: PMC2110081
- DOI: 10.1083/jcb.74.2.358
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Binding of viral glycoprotein mRNA to endoplasmic reticulum membranes is disrupted by puromycin
J Cell Biol.
1977 Aug.
Abstract
Previous studies showed that the glycoprotein (G) of vesicular stomatitis virus is synthesized in association with the endoplasmic reticulum (ER) membrane and that all G mRNA co-fractionates with ER membrane. Here, we show that treatment of infected cells with puromycin results in dissociation of G mRNA, and presumably the associated ribosomes, from the ER membrane. Even it extracts from treated cells are kept at low ionic strength (0.01 M KCl), over 80% of G mRNA is found unattached to membranes. There is no evidence for direct interaction of GmRNA with membranes; rather, the linkage apparently is mediated by the nascent G polypeptide.
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