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. 2009 Oct;53(10):4556-8.
doi: 10.1128/AAC.00395-09. Epub 2009 Jul 13.

Differential expression of ccrA in methicillin-resistant Staphylococcus aureus strains carrying staphylococcal cassette chromosome mec type II and IVa elements

Paul G Higgins  1 , Adriana E Rosato, Harald Seifert, Gordon L Archer, Hilmar Wisplinghoff
Affiliations

Differential expression of ccrA in methicillin-resistant Staphylococcus aureus strains carrying staphylococcal cassette chromosome mec type II and IVa elements

Paul G Higgins et al. Antimicrob Agents Chemother. 2009 Oct.

Abstract

Excision of staphylococcal cassette chromosome mec (SCCmec) is mediated through the ccrA- and -B-encoded recombinases. We investigated the effects of different antimicrobial agents on ccrA expression by using a ccrA::lacZ fusion and reverse transcription-PCR with methicillin (meticillin)-resistant Staphylococcus aureus strains MW2 (SCCmec IVa) and N315 (SCCmec II). Upregulation of ccrA was observed upon exposure to beta-lactam antibiotics. Vancomycin increased ccrA expression in MW2 but had no effect on N315. Vancomycin may contribute to the transfer of SCCmec IVa but have no effect in SCCmec II.

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Figures

FIG. 1.
FIG. 1.
(A) Measurement of ONPG hydrolysis with MW2-pM and N315-pN against increasing vancomycin concentrations. (B) Measurement of MW2 and N315 ccrA mRNA transcripts against increasing vancomycin concentrations.
See this image and copyright information in PMC

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