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Comparative Study
. 1977 Jun;5(6):629-34.
doi: 10.1128/jcm.5.6.629-634.1977.

Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus

H Schmitz, H W Doerr, D Kampa, A Vogt
Comparative Study

Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus

H Schmitz et al. J Clin Microbiol. 1977 Jun.

Abstract

A sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin M antibodies to cytomegalovirus is described. The results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. Horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. Nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei of cytomegalovirus-infected cells.

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References

    1. Br J Exp Pathol. 1976 Apr;57(2):243-7 - PubMed
    1. J Clin Microbiol. 1976 May;3(5):541-2 - PubMed
    1. J Med Virol. 1977;1(1):21-7 - PubMed
    1. J Immunol. 1972 Jul;109(1):129-35 - PubMed
    1. N Engl J Med. 1968 Sep 12;279(11):566-70 - PubMed

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