Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Jul;6(1):10-5.
doi: 10.1128/jcm.6.1.10-15.1977.

Solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus antibody

L A Babiuk, S D Acres, B T Rouse

Solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus antibody

L A Babiuk et al. J Clin Microbiol. 1977 Jul.

Abstract

An indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. The test involved ethanol fixation of microcultures of bovine rotavirus-infected BSC-1 cells and reacetion with bovine antirotavirus serum, followed by 125I-labeled rabbit anti-bovine immunoglobulin G. The technique was shown to be virus specific and highly sensitive. The fixed microcultures could be stored at 4 degrees C for at least 2 months without affecting the sensitivity of the test. The application of this system for the detection of rotavirus antibodies in humans is briefly discussed.

PubMed Disclaimer

References

    1. J Clin Pathol. 1976 Mar;29(3):191-7 - PubMed
    1. Vet Rec. 1975 Jan 25;96(4):85-8 - PubMed
    1. J Clin Microbiol. 1975 Mar;1(3):311-7 - PubMed
    1. J Clin Microbiol. 1975 Nov;2(5):410-8 - PubMed
    1. J Clin Microbiol. 1976 Mar;3(3):376-7 - PubMed

LinkOut - more resources