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. 2009 Jul 14:8:156.
doi: 10.1186/1475-2875-8-156.

Chloroquine and sulphadoxine-pyrimethamine sensitivity of Plasmodium falciparum parasites in a Brazilian endemic area

Bianca Ervatti Gama  1 , Natália K Almeida de Oliveira, Mariano G Zalis, José Maria de Souza, Fátima Santos, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz
Affiliations

Chloroquine and sulphadoxine-pyrimethamine sensitivity of Plasmodium falciparum parasites in a Brazilian endemic area

Bianca Ervatti Gama et al. Malar J. .

Abstract

Background: The goal of the present study was the characterization of Plasmodium falciparum genes associated to malaria drug resistance (pfcrt, pfdhfr and pfdhps), in samples from two Brazilian localities.

Methods: Parasites from 65 P. falciparum samples were genotyped using nested-PCR and direct DNA sequencing.

Results: Six resistant sulphadoxine-pyrimethamine (SP) pfdhfr genotypes and one haplotype associated to SP sensitivity were detected. For pfcrt gene, SVMNT chloroquine (CQ)-resistant genotype was detected as well as the CVMNK CQ-sensitive haplotype in the same sample from Paragominas, that showed a SP-sensitive genotype.

Conclusion: This study is the first to document the sensitivity of P. falciparum parasites to CQ and SP in Brazilian field samples. The importance of these findings is discussed.

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