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. 2009 Jul 16;51(1):30.
doi: 10.1186/1751-0147-51-30.

Intra-arterial delivery of triolein emulsion increases vascular permeability in skeletal muscles of rabbits

Hak Jin Kim  1 , Yong Woo Kim, In Sook Lee, Jong Woon Song, Yeon Joo Jeong, Seon Hee Choi, Kyung Un Choi, Kuen Tak Suh, Byung Mann Cho
Affiliations

Intra-arterial delivery of triolein emulsion increases vascular permeability in skeletal muscles of rabbits

Hak Jin Kim et al. Acta Vet Scand. .

Abstract

Background: To test the hypothesis that triolein emulsion will increase vascular permeability of skeletal muscle.

Methods: Triolein emulsion was infused into the superficial femoral artery in rabbits (triolein group, n = 12). As a control, saline was infused (saline group, n = 18). Pre- and post-contrast T1-weighted MR images were obtained two hours after infusion. The MR images were qualitatively and quantitatively evaluated by assessing the contrast enhancement of the ipsilateral muscles. Histologic examination was performed in all rabbits.

Results: The ipsilateral muscles of the rabbits in the triolein group showed contrast enhancement, as opposed to in the ipsilateral muscles of the rabbits in the saline group. The contrast enhancement of the lesions was statistically significant (p < 0.001). Histologic findings showed that most examination areas of the triolein and saline groups had a normal appearance.

Conclusion: Rabbit thigh muscle revealed significantly increased vascular permeability with triolein emulsion; this was clearly demonstrated on the postcontrast MR images.

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Figures

Figure 1
Figure 1
Pre-contrast (A) and post-contrast (B) T1-weighted axial images (TR/TE, 985/21) of a rabbit obtained 2 hours after normal saline injection into the left superficial femoral artery (saline group) revealed minimal contrast enhancement of the thigh muscles around the ipsilateral femur. The contrast enhancement is the same as that observed in the contralateral femur. White circular dots present regions of interest where signal intensity was measured in the ipsilateral and contralateral adductor magnus muscles.
Figure 2
Figure 2
Pre-contrast (A) and post-contrast (B) T1-weighted axial image (TR/TE, 985/21) of a rabbit obtained 2 hours after triolein emulsion into the left superficial femoral artery (triolein group) reveals focal enhancement of the thigh muscles posterior (adductor magnus) and lateral (vastus lateralis and vastus intermedius) to the ipsilateral femoral bone. White circular dots present regions of interest where signal intensity was measured in the ipsilateral and contralateral adductor magnus muscles.
Figure 3
Figure 3
Electron micrograph of skeletal muscle obtained from the adductor magnus of the ipsilateral thigh in a rabbit in the saline group (original magnification × 4000). An endothelial cell and an intraluminal red blood cell (RBC) is seen at the bottom of the image. Longitudinal section of the muscle shows orderly arranged A bands and Z lines. No interstitial edema or disruption of the endothelium is noted. bar: 2 μm
Figure 4
Figure 4
Electron micrograph of skeletal muscle obtained from the adductor magnus of the ipsilateral thigh in a rabbit in the triolein group (original magnification × 5000). The capillary is enlarged due to an impacted fat globule (Fat). Longitudinal section of the muscle reveals no evidence of disruption of the A bands or Z lines. Minimal disruption of the upper portion of the endothelium (black arrows) with minimal interstitial edema (white arrow) is seen. bar: 1 μm
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