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. 2009 Dec;92(6):2109-12.
doi: 10.1016/j.fertnstert.2009.05.059. Epub 2009 Jul 15.

Processing of semen can result in increased sperm DNA fragmentation

Estefanía Toro  1 , Silvia Fernández, Ana Colomar, Aïda Casanovas, Juan G Alvarez, Marisa López-Teijón, Esther Velilla
Affiliations

Processing of semen can result in increased sperm DNA fragmentation

Estefanía Toro et al. Fertil Steril. 2009 Dec.

Abstract

Processing of semen for assisted reproductive technologies (ART) entails a number of procedures that include semen liquefaction, removal of seminal plasma by centrifugation, incubation, and cryopreservation. The results of this study indicate that incubation of semen at room temperature and semen cryopreservation can result in increased levels of sperm DNA fragmentation.

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