Review
doi: 10.1016/j.bbrc.2009.07.068.
Epub 2009 Jul 17.
The removal of fluorescence in sequencing-by-synthesis
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- PMID: 19616513
- DOI: 10.1016/j.bbrc.2009.07.068
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Review
The removal of fluorescence in sequencing-by-synthesis
Biochem Biophys Res Commun.
.
Erratum in
- Biochem Biophys Res Commun. 2010 Jun 25;397(2):373
Abstract
Fluorescent oligonucleotides provide useful way to study DNA during the sequencing-by-synthesis process. They allow researchers to determine the sequence of a short DNA strand. Fluorescence created by these chemicals must be removed after a few based pairs are read in order to continue reading the DNA sequence. Researchers have developed a variety of methods to solve this problem. This paper reviews the development of fluorescence removal in DNA sequencing and summarizes several methods of removing the fluorescence.
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