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. 2009 Aug 18;101(4):589-97.
doi: 10.1038/sj.bjc.6605183. Epub 2009 Jul 21.

Detection of cytokeratin-19 mRNA-positive cells in the peripheral blood and bone marrow of patients with operable breast cancer

A Daskalaki  1 , S Agelaki, M Perraki, S Apostolaki, N Xenidis, E Stathopoulos, E Kontopodis, D Hatzidaki, D Mavroudis, V Georgoulias
Affiliations

Detection of cytokeratin-19 mRNA-positive cells in the peripheral blood and bone marrow of patients with operable breast cancer

A Daskalaki et al. Br J Cancer. .

Abstract

Background: To compare detection rates and evaluate the clinical relevance of cytokeratin-19 (CK-19) mRNA-positive cells in the peripheral blood (circulating tumour cells, CTCs) and bone marrow (disseminated tumour cells; DTCs) of patients with early breast cancer.

Methods: Paired samples of peripheral blood and bone marrow were obtained from 165 patients with stage I-II breast cancer before the initiation of adjuvant chemotherapy. In 84 patients, paired blood and bone marrow samples were also available after chemotherapy. The detection of CK-19 mRNA-positive CTCs and DTCs was assessed by real-time PCR.

Results: CK-19 mRNA-positive CTCs and DTCs were detected in 55.2 and 57.6% of patients before chemotherapy, respectively. After chemotherapy, CTCs and DTCs were identified in 44 (52.4%) and 43 (51.2%) of the 84 patients, respectively. There was a 93.9% (McNemar; P=0.344) and 72.6% (McNemar; P=0.999) concordance between blood and bone marrow samples before and after chemotherapy, respectively. The detection of CK-19 mRNA-positive CTCs or DTCs before chemotherapy was associated with decreased overall survival (P=0.024 and P=0.015, respectively). In addition, their simultaneous detection was also associated with an increased incidence of disease-related death and decreased overall survival (P=0.016).

Conclusions: The detection of CK-19 mRNA-positive CTCs using reverse transcription-PCR (RT-PCR) both before and after chemotherapy is correlated with the detection of CK-19 mRNA-positive DTCs in patients with early-stage breast cancer. The determination of the CTC status by RT-PCR conveys clinically relevant information that is not inferior to DTC status and, owing to the ease of sampling, warrants further evaluation as a tool for monitoring minimal residual disease.

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Figures

Figure 1
Figure 1
Correlation between the presence of CTCs and DTCs before initiation (A) and after the completion (B) of adjuvant chemotherapy.
Figure 2
Figure 2
Overall survival curves of patients with early breast cancer according to the detection of CTCs (A) and DTCs (B) before chemotherapy.
Figure 3
Figure 3
Overall survival curves of patients with early breast cancer according to the concomitant detection of CTCs and DTCs. (A) Both positive vs both negative. (B) Both negative vs at least one positive (‘other combinations’).
See this image and copyright information in PMC

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