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. 2009 Jul;15(7):1077-80.
doi: 10.3201/eid1507.080638.

Co-infections with chikungunya virus and dengue virus in Delhi, India

Harendra S Chahar  1 , Preeti Bharaj, Lalit Dar, Randeep Guleria, Sushil K Kabra, Shobha Broor
Affiliations

Co-infections with chikungunya virus and dengue virus in Delhi, India

Harendra S Chahar et al. Emerg Infect Dis. 2009 Jul.

Abstract

Aedes aegypti mosquitoes are common vectors for dengue virus and chikungunya virus. In areas where both viruses cocirculate, they can be transmitted together. During a dengue outbreak in Delhi in 2006, 17 of 69 serum samples were positive for chikungunya virus by reverse transcription-PCR; 6 samples were positive for both viruses.

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Figures

Figure 1
Figure 1
Agarose gel electrophoresis showing chikungunya virus (A) and dengue virus (B) PCR products. A) Lane 1, 294-bp product specific for chikungunya virus; lane 2, 100-bp DNA marker. B) Lane 1, 100-bp DNA marker; lane 2, 119-bp product specific for dengue 2 virus; lane 3, 290-bp product for dengue 3 virus and 392-bp product for dengue 4 virus.
Figure 2
Figure 2
Phylogenetic analysis of partial envelop 1 (E1) gene sequences (294 bp) of chikungunya virus strains from the 2006 dengue outbreak in Delhi, India. Neighbor-joining tree was constructed by using E1 gene sequences from various chikungunya virus sequences. O’nyong-nyong virus (AF079456) was used as an outgroup. Percentage bootstrap support is indicated by the values at each node. Delhi strains are indicated by a diamond. Scale bar indicates nucleotide substitutions per site.
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