doi: 10.1016/0021-9673(90)85053-x.
Rapid purification of cytochrome c oxidase from Paracoccus denitrificans
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- PMID: 1962788
- DOI: 10.1016/0021-9673(90)85053-x
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Rapid purification of cytochrome c oxidase from Paracoccus denitrificans
J Chromatogr.
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Abstract
Two methods are described for the purification of cytochrome c oxidase from Triton X-100 extracts of the periplasma membrane of Paracoccus denitrificans. The first is a large-scale procedure for the preparation of 100-250 nmol of cytochrome c oxidase (10-20 mg) in 1 week. The second is a rapid procedure for isolating up to 25 nmol in 2-3 days. Owing to the high yields given by fast protein liquid chromatography (FPLC) on Mono Q columns, the overall yield is about 20%, whereas the yield in many other previously published procedures does not exceed 10%. The use of FPLC on Mono Q also offers a considerable saving of time.
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