doi: 10.1073/pnas.74.6.2579.
Cholinergic metabolism and synapse formation by a rat nerve cell line
- PMID: 196294
- PMCID: PMC432217
- DOI: 10.1073/pnas.74.6.2579
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Cholinergic metabolism and synapse formation by a rat nerve cell line
Proc Natl Acad Sci U S A.
1977 Jun.
Abstract
The PC12 clone of a rat pheochromocytoma is able to synthesize acetylcholine. The amount of acetylcholine synthesized, and the specific activity of choline O-acetyltransferase (acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6), varies as a function of the culture growth curve and is dependent on cell density. The specific activity of choline acetyltransferase is increased by nerve growth factor, growth-conditioned medium from a variety of cell types, and adenosine 3':5'-monophosphate. Finally, the PC12 cell line is able to form cholinergic synapses with a clonal cell line of skeletal muscle origin.
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