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Review
. 1990 Oct;108(4):505-6.
doi: 10.1093/oxfordjournals.jbchem.a123229.

Human plasma gelsolin binds adenosine triphosphate

H Yamamoto  1 , H Ito, H Nakamura, E Hayashi, S Kishimoto, T Hashimoto, K Tagawa
Affiliations
Free article
Review

Human plasma gelsolin binds adenosine triphosphate

H Yamamoto et al. J Biochem. 1990 Oct.
Free article

Abstract

Binding studies of human plasma gelsolin with ATP were done by equilibrium dialysis. Analysis of the binding data showed that plasma gelsolin had one class of ATP binding site with Kd = 2.8 x 10(-7) M, which saturated at an ATP/gelsolin ratio of 0.6. The bioluminescent assay for ATP with luciferin and firefly luciferase confirmed that the protein contained a nucleotide as ATP.

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