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. 2009 Oct;79(1):121-3.
doi: 10.1016/j.mimet.2009.07.015. Epub 2009 Jul 25.

Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay

Qing Qu  1 , Ziwen Zhu, Yufei Wang, Zhijun Zhong, Jin Zhao, Feng Qiao, Xinying Du, Zhoujia Wang, Ruifu Yang, Liuyu Huang, Yaqin Yu, Lei Zhou, Zeliang Chen
Affiliations

Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay

Qing Qu et al. J Microbiol Methods. 2009 Oct.

Abstract

A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5 x 10(6)CFU/ml and the sensitivity for different spiked samples ranged from 2.0 x 10(3) to 3.9 x 10(5)CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection.

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