doi: 10.1016/j.mimet.2009.07.015.
Epub 2009 Jul 25.
Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay
Affiliations
- PMID: 19635504
- DOI: 10.1016/j.mimet.2009.07.015
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Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay
J Microbiol Methods.
2009 Oct.
Abstract
A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5 x 10(6)CFU/ml and the sensitivity for different spiked samples ranged from 2.0 x 10(3) to 3.9 x 10(5)CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection.
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