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Review
. 2009 Oct 2;284(40):27031-5.
doi: 10.1074/jbc.R109.040535. Epub 2009 Jul 27.

Aspects of the control of phosphoenolpyruvate carboxykinase gene transcription

Jianqi Yang  1 , Lea Reshef, Hanoch Cassuto, Gabriela Aleman, Richard W Hanson
Affiliations
Review

Aspects of the control of phosphoenolpyruvate carboxykinase gene transcription

Jianqi Yang et al. J Biol Chem. .
No abstract available

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Figures

FIGURE 1.
FIGURE 1.
Transcriptional regulation of the rat PEPCK-C gene promoter. A, schematic illustration of interaction between transcription factors with their transcription factor-binding sites in the rat PEPCK-C gene promoter. The figure is modified from a drawing by Chakravarty et al. (13) and is in scale. Some small transcription factor-binding sites, which overlapped larger transcription factor-binding sites, are shown as yellow bars. The names of transcription factor-binding sites, which were identified using in silico analyses, are marked in red. B, the cAMP regulatory unit (6), the acidosis regulatory unit (35), and the extended GRU in the PEPCK-C gene promoter (9). Specific transcription factors, binding sites, and co-activators are highlighted. C, transcription factor-binding sites involved in tissue-specific regulation of PEPCK-C gene transcription. COUP, chicken ovalbumin upstream promoter; C/EBP, CAAT/enhancer-binding protein; CREB, cAMP response element-binding protein; GR, glucocorticoid receptor; T3R, thyroid hormone receptor; NF1, nuclear factor 1; RAR/RXR, retinoic acid receptor/retinoid X receptor; TRE, thyroid hormone regulatory element; CBP, CREB-binding protein; IRS, insulin-responsive sequence. P1–P6 are protein-binding sites identified by DNase I footprinting (31).
FIGURE 2.
FIGURE 2.
Sequence conservation in the rat PEPCK-C gene (Pck1). A, the sequence of the rat PEPCK-C gene (−7/+7 kb) was aligned with the counterparts of eight vertebrates (mouse, human, dog, cow, opossum, chicken, frog, and zebrafish) using the UCSC Genome Bioinformatics browser. The transcription start site is marked as +1. Exons are depicted as black boxes, with numbered introns in between. Evolutionarily conserved sequences for each species listed are displayed in green. Pairwise alignments of each species with the rat genome are displayed as blue peaks below the gene. DNase I-hypersensitive (HS) sites, reported by Ip et al. (36) and by Cissell and Chalkley (23), are mapped using vertical red dashed lines. Genome assemblies used are rat (November 2004, rn4), mouse (February 2006, mm8), human (March 2006, hg18), dog (May 2005, canFam2), cow (March 2005, bosTau2), opossum (January 2006, monDom4), chicken (February 2004, galGal2), frog (October 2004, xenTro1), and zebrafish (May 2005, danRer3). B, transcription factor-binding sites in rat PEPCK-C gene promoter (−1500/+72) were aligned with the pairwise alignments track in A. The degree of sequence conservation is denoted by the blue peaks, and the conserved transcription factor-binding sites located in the PEPCK-C gene promoter are indicated at the bottom (the drawing is in scale). GR, glucocorticoid receptor; C/EBP, CAAT/enhancer-binding protein; CRE, cAMP response element.
See this image and copyright information in PMC

References

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