The reversible cross-linking of receptors by ligands: theory for the prediction of binding responses

Abstract

The general concept of receptor aggregation through the action of a cross-linking ligand is considered, three models being examined in detail. In two, ligand self-interaction leads to the formation of receptor cross-links whereas, in the third, receptor cross-links are formed through a single ligand bridge. Binding equations in closed form are formulated for each case and are shown to predict both concave and convex Scatchard plots in binding studies conducted with such systems. The significant point which emerges is that while each of the systems gives rise to very different types of binding responses, the form of the binding response is always strongly dependent on the total concentration of receptor in the system.