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. 2009 Jul;60(1-3):55.
doi: 10.1007/s10616-009-9214-z. Epub 2009 Jul 30.

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

Serkan Yılmaz  1 , Fatma Ünal  2 , Deniz Yüzbaşıoğlu  2
Affiliations

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

Serkan Yılmaz et al. Cytotechnology. 2009 Jul.

Abstract

The chromosomal aberration (CA), sister chromatid exchange (SCE) and micronucleus test (MN) were employed to investigate the in vitro effect of antimicrobial food additive benzoic acid on human chromosomes. Lymphocytes were incubated with various concentrations (50, 100, 200 and 500 μg/mL) of benzoic acid. The results of used assays showed that benzoic acid significantly increased the chromosomal aberration, sister chromatid exchange and micronucleus frequency (200 and 500 μg/mL) without changing the pH of the medium in a dose-dependent manner. Also this additive significantly decreased the mitotic index (MI) at the highest concentration for 24 h and 100, 200 and 500 μg/mL for 48 h. This decrease was dose-dependent as well. However, it did not effect the replication (RI) and nuclear division (NDI) indices.

Keywords: Benzoic acid; Chromosome aberrations; Food additive; Human lymphocytes; Micronucleus test; Sister chromatid exchanges.

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