[Study on the enrichment and purification of total flavonoids in Microcos paniculata by macroporous adsorption resin]

Abstract

Objective: To establish an optimum enrichment and purification process of total flavonoids in Microcos paniculata by macroporous resins.

Methods: Five kinds of resins were compared and the best one was chosen. Then the parameters of the process were optimized by single factor tests, uniform design and statistical methods.

Results: DI01 was selected for its excellent adsorption and desorption properties, 70% ethanol was found to be the best elution solution. As far as the yield was considered, the best result was based on the followings: feed rate-1.0 BV/h, elution flow rate-2.0 BV/h, sample concentration-7.88 mg/mL, eluting agent amount-2.0 BV, pH value 4.8; then the yield reached 90.18% and the purity was 54.37%. If the purity was considered, the best parameters wereas follows: feed rate-1.0 BV/h, elution flow rate-2.0 BV/h, sample concentration-2.0 mg/mL, eluting agent amount-2.8 BV, pH value 7.8; then the purity reached 61.77% and the yield was 80.25%.

Conclusion: The total flavonoids of Microcos paniculata can be effectively purificated and separated by D101 macroporous resin.