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. 2009 Jul 31;2(1):35.
doi: 10.1186/1756-3305-2-35.

Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs (ORESTES)

Carolina R Almeida  1 , Patricia H Stoco, Glauber Wagner, Thaís Cm Sincero, Gianinna Rotava, Ethel Bayer-Santos, Juliana B Rodrigues, Maísa M Sperandio, Antônio Am Maia, Elida Pb Ojopi, Arnaldo Zaha, Henrique B Ferreira, Kevin M Tyler, Alberto Mr Dávila, Edmundo C Grisard, Emmanuel Dias-Neto
Affiliations

Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs (ORESTES)

Carolina R Almeida et al. Parasit Vectors. .

Abstract

Background: Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerci larval form.

Results: Using Expressed Sequence Tags (ESTs) produced by the ORESTES method, a total of 1,520 high quality ESTs were generated from 20 ORESTES cDNA mini-libraries and its analysis revealed fragments of genes with promising applications including 51 ESTs matching antigens previously described in other species, as well as 113 sequences representing proteins with potential extracellular localization, with obvious applications for immune-diagnosis or vaccine development.

Conclusion: The set of sequences described here will contribute to deciphering the expression profile of this important parasite and will be informative for the genome assembly and annotation, as well as for studies of intra- and inter-specific sequence variability. Genes of interest for developing new diagnostic and therapeutic tools are described and discussed.

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Figures

Figure 1
Figure 1
Curated Gene Ontology categorization of the Taenia solium transcriptomic sequences. Graphs shows the number of T. solium ORESTES sequences with curated Gene Ontology categorization of the distinct ontologies, Biological Process, Molecular Function and Cellular Component.
Figure 2
Figure 2
(A) Distribution of the Taenia solium sequences according to the Gene Ontology sub-categorization concerning Biological Process. The predominant category of the Biological Process ontology was Cellular Process in T. solium ORESTES sequences. (B) Distribution of the Taenia solium sequences according to Gene Ontology sub-categorization Molecular Function. Among the Molecular Function Ontology it was possible to attribute the Binding and Catalytic activity function terms to sets of sequences, indicative of a close relationship between host and parasite gene regulation during the process of cyst establishment and the host response to infection.
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