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. 2009 Jul 31:15:1485-91.

Evaluation of lenticular antioxidant and redox system components in the lenses of acetyl-L-carnitine treatment in BSO-induced glutathione deprivation

R Elanchezhian  1 , M Sakthivel, M Isai, P Geraldine, P A Thomas
Affiliations

Evaluation of lenticular antioxidant and redox system components in the lenses of acetyl-L-carnitine treatment in BSO-induced glutathione deprivation

R Elanchezhian et al. Mol Vis. .

Abstract

Purpose: To investigate whether acetyl-L-carnitine (ALCAR) retards L-buthionine-(S,R)-sulfoximine (BSO)-induced cataractogenesis in Wistar rat pups.

Methods: On postpartum day 3, group I pups received intraperitoneal (ip) saline and group II and group III pups received i.p. injections of BSO once daily for three consecutive days. In addition, group III pups received ip ALCAR once daily from postpartum days 3-15. Both eyes of each pup were examined up from postpartum day 16 to day 30. After sacrifice, extricated pup lenses were analyzed for antioxidant and redox system components.

Results: There was dense lenticular opacification in all group II pups, minimal opacification in 40% of group III pups, and no opacification in 60% of group III pups and in all of group I pups. Group II lenses exhibited significantly lower values of antioxidant and redox system components and higher malondialdehyde concentrations than in group I or group III lenses.

Conclusions: ALCAR prevents cataractogenesis in the BSO-induced cataract model, possibly by inhibiting depleting antioxidant enzyme and redox system components and inhibiting lipid peroxidation.

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Figures

Figure 1
Figure 1
Slit-lamp appearance of the eye of a 30-day-old Wistar rat pup in group II. The eye exhibited dense opacification of the lens (Grade +++ opacification).
Figure 2
Figure 2
Slit-lamp appearance of the eye of 30-day-old Wistar rat pup in group III. This eye exhibited only slight opacification of the lens (Grade + opacification).
Figure 3
Figure 3
Slit-lamp appearance of the eye of 30-day-old Wistar rat pup in group I. This eye exhibited no opacification of the lens (Grade 0 opacification).
Figure 4
Figure 4
Concentration of malondialdehyde in lenses of 30-day-old Wistar rat pups. Values are expressed as mean±SD (n=5). An asterisk indicates that a significant difference was found between group I and group II values (p≤0.05). The sharp (hash mark) indicates that a significant difference was found between group II and group III values (p≤0.05).
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