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. 1990 Dec;40(4):323-9.
doi: 10.1016/0162-0134(90)80066-7.

Effect of ascorbic acid, sodium citrate, sodium bicarbonate, and their combination effect on the binding of 59Fe2+ and 59Fe3+ to plasma membrane of lactating mouse mammary gland

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Effect of ascorbic acid, sodium citrate, sodium bicarbonate, and their combination effect on the binding of 59Fe2+ and 59Fe3+ to plasma membrane of lactating mouse mammary gland

D A Moutafchiev et al. J Inorg Biochem. 1990 Dec.

Abstract

Isolated plasma membranes of lactating mouse mammary gland were treated with different concentrations of ascorbate, sodium citrate, sodium bicarbonate, and combinations of them (from 16 x 10(-10) to 4 x 10(-6) moles/L) and studied for the binding of 59Fe2+ and 59Fe3+ at pH 7.4. The results show that the Fe3+ form of iron is under a greater influence of anions used in these experiments. The Fe2+ form of iron is weakly bounded and affected. It is suggested that the form with a greater positive electric charge is more effectively bound to the receptors in plasma membranes.

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