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. 2010 Jan;37(1):473-6.
doi: 10.1007/s11033-009-9654-8. Epub 2009 Aug 2.

A new SNP in the 3'UTR region of the bovine calpain small subunit (CAPNS1) gene

E Juszczuk-Kubiak  1 , K Flisikowski, K Wicińska
Affiliations

A new SNP in the 3'UTR region of the bovine calpain small subunit (CAPNS1) gene

E Juszczuk-Kubiak et al. Mol Biol Rep. 2010 Jan.

Abstract

Calpains are a ubiquitous cytoplasmic cysteine protease, the activity of which is absolutely dependent on calcium. This proteolytic system degrades myofibrillar protein under post-mortem conditions and appears to be the primary enzyme in the postmortem tenderization process. In the present study a new single nucleotide polymorphism was found in the bovine CAPNS1 gene exon 11 coding for the 3'UTR. Transition C --> T at position 6536 was detected and identified using PCR-SSCP and DNA sequencing techniques, and then analysed with PCR-RFLP using MboII nuclease. The genotype frequencies and alleles distribution were studied in 190 bulls including, Charolaise, Hereford, Limousine, Simmental, Polish Red and Fresian breeds.

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