Lysinibacillus xylanilyticus sp. nov., a xylan-degrading bacterium isolated from forest humus

Abstract

A novel xylan-degrading bacterium, designated XDB9(T), was isolated from forest humus collected from Gyeryong Mountain in Korea. Cells were Gram-positive, aerobic, motile and endospore-forming rods. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain XDB9(T) was most closely related to members of the genus Lysinibacillus. 16S rRNA gene sequence similarities between strain XDB9(T) and the type strains of species of the genus Lysinibacillus ranged from 98.0 to 98.5 %. The cell-wall peptidoglycan type of strain XDB9(T) was A4alpha, which is based on L-Lys-D-Asp. Strain XDB9(T) contained iso-C(15 : 0) and C(16 : 1)omega7c alcohol as the major fatty acids and MK-7 as the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 37.2 mol%. The DNA-DNA hybridization results and differential phenotypic properties showed that strain XDB9(T) could be distinguished from recognized species of the genus Lysinibacillus. It was concluded that strain XDB9(T) represents a new taxon for which the name Lysinibacillus xylanilyticus sp. nov. is proposed. The type strain is XDB9(T) (=KCTC 13423(T)=CCUG 57438(T)).