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. 1990 Nov;14(4):223-9.
doi: 10.1007/BF00429889.

Detection of free cytoplasmic circles of transposon Tn9 multimers in Escherichia coli

J F Sarkari  1 , S K Mahajan
Affiliations

Detection of free cytoplasmic circles of transposon Tn9 multimers in Escherichia coli

J F Sarkari et al. Mol Biol Rep. 1990 Nov.

Abstract

Extrachromosomal circular DNA molecules consisting of IS1-cat repeats, (IS1-cat)n, were isolated from an E. coli strain harboring nearly 30 copies of tandemly amplified transposon Tn9 located on the chromosome. The DNA 'circles' were characterized by restriction analysis followed by Southern blotting and electron microscopic examination. Their size varied from approximately 5.5 kb to 53 kb.

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