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. 1977 May 13;113(1-2):159-61.
doi: 10.1007/BF00428597.

Location of three key enzymes of gluconeogenesis in baker's yeast

S Haarasilta, L Taskinen

Location of three key enzymes of gluconeogenesis in baker's yeast

S Haarasilta et al. Arch Microbiol. .

Abstract

The subcellular location of hexose diphosphatase, phosphoenolpyruvate carboxykinase and pyruvate carboxylase in baker's yeast (Saccharomyces cerevisiae) was investigated by density gradient centrifugation of spheroplast lysates obtained by osmotic shock treatment of spheroplasts and centrifugation for 10000 g x min. On the evidence obtained from zonal separations these three enzymes of gluconeogenesis are most probably located in the soluble cytosol.

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