Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra)

Abstract

A novel strain, designated TP2-8(T), was isolated from fermented fish (pla-ra) in Thailand. It stained Gram-positive and the cells were aerobic, endospore-forming rods. The strain grew at pH 6-8 (optimum pH 7), 15-55 degrees C (optimum 37 degrees C) and 1-22 % (w/v) NaCl (optimum 5-10 %). It contained meso-diaminopimelic in the cell-wall peptidoglycan. MK-7 and cellular fatty acids anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0) were major components. Polar lipids diphosphatidylglycerol and phosphatidylglycerol and unidentified lipids were detected. The DNA G+C content was 37.6 mol%. Comparison of the 16S rRNA gene sequence of strain TP2-8(T) with those of other members of the family Bacillaceae indicated that it was a member of the genus Gracilibacillus (94.9-99.2 % sequence similarity) and was closely related to Gracilibacillus saliphilus YIM 91119(T) (99.2 % similarity), G. lacisalsi BH312(T) (98.6 %), G. orientalis XH-63(T) (97.7 %), 'G. quinghaiensis' YIM C229 (97.7 %) and G. boraciitolerans T-16X(T) (97.2 %). Strain TP2-8(T) showed low DNA-DNA relatedness (< or = 49%) to G. saliphilus YIM 91119(T), G. lacisalsi DSM 19029(T), G. orientalis CCM 7326(T), 'G. quinghaiensis' DSM 17858 and G. boraciitolerans JCM 21714(T). On the basis of the physiological and biochemical characteristics and molecular data presented, strain TP2-8(T) is proposed to represent a novel species, Gracilibacillus thailandensis sp. nov. (type strain TP2-8(T) =JCM 15569(T) =PCU 304(T) =TISTR 1881(T)).