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. 1990 May;14(5):881-8.
doi: 10.1007/BF00016525.

The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis

W Y Cheung  1 , M D Gale
Affiliations

The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis

W Y Cheung et al. Plant Mol Biol. 1990 May.

Abstract

A method is presented for the preparation of large DNA molecules from protoplasts embedded in agarose blocks of three different cereals--hexaploid bread wheat (Triticum aestivum), barley (Hordeum vulgare) and rye (Secale cereale). Pulse-field gel electrophoresis (PFGE) analysis of these DNA preparations using a contour-clamped homogeneous field (CHEF) apparatus indicated that the size of the DNA molecules was greater than 6 Mb. DNA samples prepared by this method were shown to be useful for restriction analysis using both frequent and rare cutting enzymes.

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References

    1. Cell. 1984 May;37(1):67-75 - PubMed
    1. Methods Enzymol. 1987;151:461-89 - PubMed
    1. Nucleic Acids Res. 1989 Apr 11;17(7):2801-18 - PubMed
    1. Anal Biochem. 1984 Feb;137(1):266-7 - PubMed
    1. Science. 1987 May 15;236(4803):806-12 - PubMed

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