Yaba virus-specific DNA in the host cell nucleus

Abstract

DNA-DNA hybridization studies show that Yaba virus-specific DNA is present in the host cell nucleus late in the infection cycle. The nuclear DNA appears to exist as a complete genome, not convalently linked to host cell DNA, as demonstrated by sedimentation analyses. The DNA apperas to be synthesized in the nucleus, since its level of incorporation of label is ten times the background incorporation detectable in the cytoplasm. Extraction of the nuclei by treatment with SDS and EDTA after precipitation with 1 M NaCl separates most of cellular DNA from the Yaba virus-specific DNA.