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. 1990 Sep;15(3):457-64.
doi: 10.1007/BF00019162.

Partial purification and characterization of a DNA helicase from chloroplasts of Glycine max

G C Cannon  1 , S Heinhorst
Affiliations

Partial purification and characterization of a DNA helicase from chloroplasts of Glycine max

G C Cannon et al. Plant Mol Biol. 1990 Sep.

Abstract

A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg2+ or Mn2+. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure.

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