Rec mutants of Escherichia coli deficient in subunits of rec BC (D) complex
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- PMID: 1966645
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Rec mutants of Escherichia coli deficient in subunits of rec BC (D) complex
Acta Biochim Biophys Hung.
1990.
Abstract
The inactivation of rec BC (D) DNase upon chromatography on DEAE-cellulose was observed. Simultaneously DNA-stimulated ATPases (I and II) and DNase activities on single- and double-stranded DNA substrates were measured in Escherichia coli rec+ and rec- cell extracts. Normal levels of ATPase I and II were detected in rec+ cells. Rec A- cells were lacking DNA dependent ATPase I, while rec B single and rec BC double mutants were defective in DNA dependent ATPase II, the second major enzyme of this type. Rec B and C mutations did not change DNase activities. Rec A mutation significantly increased DNase activity on linear single-stranded substrate.
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