Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Sep;6(9):639-41.
doi: 10.1038/nmeth.1361. Epub 2009 Aug 9.

Accurate determination of microbial diversity from 454 pyrosequencing data

Christopher Quince  1 , Anders Lanzén, Thomas P Curtis, Russell J Davenport, Neil Hall, Ian M Head, L Fiona Read, William T Sloan
Affiliations

Accurate determination of microbial diversity from 454 pyrosequencing data

Christopher Quince et al. Nat Methods. 2009 Sep.

Abstract

We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.

PubMed Disclaimer

Comment in

References

    1. Pac Symp Biocomput. 2008;:75-86 - PubMed
    1. Appl Environ Microbiol. 2005 Mar;71(3):1501-6 - PubMed
    1. Microbiology (Reading). 1996 May;142 ( Pt 5):1107-1114 - PubMed
    1. Nature. 2005 Sep 15;437(7057):376-80 - PubMed
    1. Science. 2007 Oct 5;318(5847):97-100 - PubMed

Publication types