Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Sep;6(9):647-9.
doi: 10.1038/nmeth.1360. Epub 2009 Aug 9.

Digital transcriptome profiling using selective hexamer priming for cDNA synthesis

Christopher D Armour  1 , John C Castle, Ronghua Chen, Tomas Babak, Patrick Loerch, Stuart Jackson, Jyoti K Shah, John Dey, Carol A Rohl, Jason M Johnson, Christopher K Raymond
Affiliations

Digital transcriptome profiling using selective hexamer priming for cDNA synthesis

Christopher D Armour et al. Nat Methods. 2009 Sep.

Abstract

We developed a procedure for the preparation of whole transcriptome cDNA libraries depleted of ribosomal RNA from only 1 microg of total RNA. The method relies on a collection of short, computationally selected oligonucleotides, called 'not-so-random' (NSR) primers, to obtain full-length, strand-specific representation of nonribosomal RNA transcripts. In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing.

PubMed Disclaimer

References

    1. J Microbiol Methods. 2007 Dec;71(3):288-91 - PubMed
    1. Curr Biol. 2008 Nov 25;18(22):1735-41 - PubMed
    1. Nucleic Acids Res. 2005 Jan 1;33(Database issue):D501-4 - PubMed
    1. Nature. 2008 Nov 27;456(7221):470-6 - PubMed
    1. Nat Methods. 2008 Jul;5(7):613-9 - PubMed