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. 2008 Sep;2(3):581-4.
doi: 10.2147/opth.s2643.

Quantitative determination of glycosaminoglycans in tears of diabetic patients

Marilita M Moschos  1 , Alexandros A Rouvas, Spyridon Papadimitriou, Athanasios Kotsolis, Nikolaos Sitaras, Michael Apostolopoulos
Affiliations
Free PMC article

Quantitative determination of glycosaminoglycans in tears of diabetic patients

Marilita M Moschos et al. Clin Ophthalmol. 2008 Sep.
Free PMC article

Abstract

Purpose: To determine the amount of glycosaminoglycans (GAGs) in tears of patients with diabetic retinopathy (DR) and to compare it with normal subjects.

Methods: 38 patients with DR and 24 normal volunteers were included. Thirty subjects suffered from background diabetic retinopathy (BDR) and 8 from proliferate diabetic retinopathy (PDR). For the GAGs assay, the uronic carbazole reaction was used.

Results: The mean concentration of GAGs was significantly higher in patients with DR than in normal subjects. The GAGs concentration in patients with BDR or PDR was significantly higher than in normal subjects.

Conclusion: The measurement of GAGs in tears of diabetic patients could be a tool in order to assess the stability or not of the disease.

Keywords: diabetic retinopathy; glycosaminoglycans; tears.

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Figures

Figure 1
Figure 1
Graph showing the mean concentration of GAGs in tears of patients with BDR and normal controls (p < 0.05).
Figure 2
Figure 2
Graph showing the mean concentration of GAGs in tears of patients with PDR and normal individuals (p > 0.05).
See this image and copyright information in PMC

References

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