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. 2010 Apr;37(4):2111-5.
doi: 10.1007/s11033-009-9676-2. Epub 2009 Aug 9.

Pro-inflammatory cytokines of rat vasculature in DOCA-salt treatment

Behjat Seifi  1 , Mehri Kadkhodaee, Jie Xu, Manoocher Soleimani
Affiliations

Pro-inflammatory cytokines of rat vasculature in DOCA-salt treatment

Behjat Seifi et al. Mol Biol Rep. 2010 Apr.

Abstract

The purpose of this study was to ascertain the onset expression of pro-inflammatory cytokines in the aorta and kidney and establish their correlation with the increase in arterial blood pressure in rats subjected to DOCA-salt treatment. Male Sprague-Dawley rats underwent unilateral nephrectomy and received subcutaneous DOCA (20 mg/rat/week) as well as 1% NaCl and 0.2% KCl for drinking for 2 weeks. Blood pressure and expression of pro-inflammatory cytokines in aorta and kidney were studied weekly during the induction of hypertension. The treated rats exhibited a mild elevation of blood pressure at 1 week and a profound increase at 2 weeks. Quantitative RT-PCR demonstrated a 4.9-fold and a 3.6-fold enhancement in the expression of TNF-alpha and IL-6, respectively, in aorta as early as 1 week. The expression of IL-6 and TNF-alpha in the kidney remained almost unchanged at 1 week but mildly increased at 2 weeks DOCA-salt treatment. This study indicates a robust increase in the expression of IL-6 and TNF-alpha in aorta in DOCA-salt treated rats. This enhancement suggests that the activation of pro-inflammatory cytokines may contribute to onset of the elevation of blood pressure in DOCA-salt hypertension model.

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Figures

Fig. 1
Fig. 1
Time course of systolic blood pressure elevation in rats on DOCA-salt treatment. The data are presented as mean ± SEM. * P < 0.05 compared to sham-operated, # P < 0.05 compared to first week
Fig. 2
Fig. 2
Expression of IL-6 and TNF-α in aorta of rats on DOCA and/or salt treatment. a IL-6 and TNF-α expression by RT-PCR in DOCA-salt treatment. b IL-6 and TNF-α expression by RT-PCR in DOCA or salt treatment. c Fold change in the expression of IL-6 and TNF-α in DOCA-salt treatment
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