Review
doi: 10.1016/j.diagmicrobio.2009.04.015.
A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis
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- PMID: 19679229
- DOI: 10.1016/j.diagmicrobio.2009.04.015
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Review
A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis
Diagn Microbiol Infect Dis.
2009 Sep.
Abstract
Yersinia pestis isolates were genotyped analyzing the polymorphic DNA regions named variable number tandem repeats (VNTR). Allele variants were studied by high-resolution melting analysis (HRMA) of polymerase chain reaction fragments obtained for 25 VNTR loci. After comparison with previous results, 14 loci gave distinguishable normalized melting curves and allowed to correctly assign alleles. This HRMA typing technique permits to differentiate Y. pestis isolates and turned out to be robust, reproducible, and cheap.
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